Characterization of the spontaneous elimination of streptomycin sensitivity (SmS) on high-copy-number plasmids: SmS-enforcement cloning vectors with a synthetic rpsL gene.

doi:10.1016/0378-1119(92)90158-l

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Article

Characterization of the spontaneous elimination of streptomycin sensitivity (SmS) on high-copy-number plasmids: SmS-enforcement cloning vectors with a synthetic rpsL gene.

Gene - 2 Nov 1992

Toba-Minowa M, Hashimoto-Gotoh T

Abstract excerpt

The strAS or rpsL+ gene, encoding a ribosomal protein, S12, expresses its streptomycin-sensitivity (SmS) phenotype dominantly over strAR or rpsL- gene. Therefore, strAR cells that harbor plasmids with strAS alleles are phenotypically SmS. It was found that the SmS phenotype is unstable, and such...

Topics

Amino Acid Sequence
Base Sequence
Cloning, Molecular
Drug Resistance, Microbial
Escherichia coli
Escherichia coli Proteins
Genes, Synthetic
Genetic Vectors
Molecular Sequence Data
Phenotype
Plasmids
Restriction Mapping
Ribosomal Protein S9
Ribosomal Proteins
Streptomycin
Transformation, Genetic

Source

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Identifiers

PMID: 1427096
DOI: 10.1016/0378-1119(92)90158-l

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-1427096-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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