pKSS--a second-generation general purpose cloning vector for efficient positive selection of recombinant clones.

doi:10.1016/0378-1119(94)90790-0

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Article

pKSS--a second-generation general purpose cloning vector for efficient positive selection of recombinant clones.

Gene - 28 Jan 1994

Kast P

Abstract excerpt

A new small plasmid vector (pKSS) for the direct selection of insert-containing plasmid clones is presented. The selection strategy is based on the acquired sensitivity of Escherichia coli cells to p-chloro-phenylalanine (p-Cl-Phe) if they carry a pheS allele encoding a phenylalanyl-tRNA syntheta...

Topics

Alleles
Base Sequence
Cloning, Molecular
Escherichia coli
Fenclonine
Genetic Vectors
Molecular Sequence Data
Phenylalanine-tRNA Ligase
Plasmids
Recombinant Fusion Proteins
Recombinant Proteins
Restriction Mapping
Substrate Specificity
beta-Galactosidase

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