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Article

Genetic analysis and overexpression of lipolytic activity in Bacillus subtilis.

Applied and environmental microbiology - 1 May 1994

Dartois V, Coppée J Y, Colson C, Baulard A

Abstract excerpt

The previously cloned Bacillus subtilis lipase gene (lip) was mapped on the chromosome and used in the construction of a B. subtilis derivative totally devoid of any lip sequence. Homologous overexpression was performed in this strain by subcloning the lip open reading frame on a multicopy plasmid under the control of a strong gram-positive promoter. A 100-fold overproducing strain was obtained, which should...

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Identifiers

PMID: 8017946
DOI: 10.1128/aem.60.5.1670-1673.1994
PMCID: PMC201535

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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