Genetic analysis and overexpression of lipolytic activity in Bacillus subtilis.

doi:10.1128/aem.60.5.1670-1673.1994

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Article

Genetic analysis and overexpression of lipolytic activity in Bacillus subtilis.

Applied and environmental microbiology - 1 May 1994

Dartois V, Coppée J Y, Colson C, Baulard A

Abstract excerpt

The previously cloned Bacillus subtilis lipase gene (lip) was mapped on the chromosome and used in the construction of a B. subtilis derivative totally devoid of any lip sequence. Homologous overexpression was performed in this strain by subcloning the lip open reading frame on a multicopy plasmi...

Topics

Alleles
Bacillus subtilis
Chromosome Mapping
Chromosomes, Bacterial
Gene Expression
Lipase
Plasmids

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