Article
Identification of a gene in Bacillus subtilis bacteriophage SPP1 determining the amount of packaged DNA.
Journal of molecular biology - 5 May 1992
Tavares P, Santos M A, Lurz R, Morelli G, de Lencastre H, Trautner T A
Abstract excerpt
The virulent Bacillus subtilis bacteriophage SPP1 encapsidates its DNA by a headful mechanism. Analyzing phage missense mutants, which package less DNA than SPP1 wild-type but show no other affected properties, we have identified a gene whose product is involved in the sizing of phage DNA during maturation. Characterization of this gene and its product provides an experimental access to the poorly understood...
Topics
- Amino Acid Sequence
- Bacillus subtilis
- Bacteriophages
- Base Sequence
- Cloning, Molecular
- DNA, Viral
- Genes, Viral
- Genetic Complementation Test
- Microscopy, Electron
- Molecular Sequence Data
- Mutation