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Article

Stable positional cloning of long continuous DNA in the Bacillus subtilis genome vector.

Journal of biochemistry - 1 Oct 2003

Itaya Mitsuhiro, Fujita Kyoko, Ikeuchi Masahiko, Koizumi Maki, Tsuge Kenji

Abstract excerpt

Direct cloning of a long continuous genome segment in a Bacillus subtilis genome vector was demonstrated for the first time. Two small DNA fragments had to be installed in the vector prior to cloning. The DNA between these two fragments was cloned via homologous recombination. The efficiency of cloning was estimated using the 3,573-kb genome of a cyanobacterium, Synechocystis sp. PCC 6803. Recombinants were...

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Identifiers

PMID: 14607977
DOI: 10.1093/jb/mvg168

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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