Kinetic and equilibrium alpha-secondary tritium isotope effects on reactions catalyzed by dCMP hydroxymethylase from bacteriophage T4.

doi:10.1021/bi00248a014

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Article

Kinetic and equilibrium alpha-secondary tritium isotope effects on reactions catalyzed by dCMP hydroxymethylase from bacteriophage T4.

Biochemistry - 8 Nov 1994

Graves K L, Hardy L W

Abstract excerpt

Deoxycytidylate (dCMP) hydroxymethylase (CH) catalyzes the formation of 5-(hydroxymethyl)-dCMP, essential for DNA synthesis in phage T4, from dCMP and methylenetetrahydrofolate (CH2THF). The nucleotide analog 5-fluorodeoxuridylate (FdUMP) stoichiometrically inactivates CH by formation of a covale...

Topics

Bacteriophage T4
Catalysis
Electrophoresis, Polyacrylamide Gel
Escherichia coli
Fluorodeoxyuridylate
Hydroxymethyl and Formyl Transferases
Kinetics
Mutation
Protein Binding
Transferases
Tritium

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