Mutations in human interferon gamma affecting inclusion body formation identified by a general immunochemical screen.

doi:10.1038/nbt0891-731

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Article

Mutations in human interferon gamma affecting inclusion body formation identified by a general immunochemical screen.

Bio/technology (Nature Publishing Company) - 1 Aug 1991

Wetzel R, Perry L J, Veilleux C

Abstract excerpt

High level expression of the gene for human interferon-gamma (HuIFN-gamma) in E. coli JM101 cultured at 37 degrees C results in the distribution of over 90 percent of the total accumulated gene product into inclusion bodies (IBs). We have identified mutations throughout the molecule that alter th...

Topics

Amino Acid Sequence
Cell Compartmentation
Cloning, Molecular
Cytoplasm
Escherichia coli
Guanidine
Guanidines
Immunoassay
Immunochemistry
Interferon-gamma
Molecular Sequence Data
Mutation
Solubility

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