Article
Validity of an ELISA for N-acetyltransferase-2 (NAT2) phenotyping.
Journal of immunological methods - 1 May 2001
Wong P, Banerjee K, Massengill J, Nowell S, Lang N, Leyland-Jones B
Abstract excerpt
A competitive antigen ELISA was previously developed for NAT2 phenotyping, using caffeine as the probe drug. The ELISA phenotypes by measuring the ratio of 5-acetamido-6-amino-3-methyluracil (AAMU) and 1-methylxanthine (1X) after transformation of 5-acetamido-6-formylamino-3-methyluracil (AFMU) to AAMU, in contrast to capillary electrophoresis high-pressure liquid chromatography (HPLC) which phenotype by...
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