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Article

Conformational changes and cleavage by the homing endonuclease I-PpoI: a critical role for a leucine residue in the active site.

Journal of molecular biology - 21 Jul 2000

Galburt E A, Chadsey M S, Jurica M S, Chevalier B S, Erho D, Tang W, Monnat R J, Stoddard B L

Abstract excerpt

The homing endonuclease I-PpoI severely bends its DNA target, resulting in significant deformations of the minor and major groove near the scissile phosphate groups. To study the role of conformational changes within the protein catalyst and the DNA substrate, we have determined the structure of the enzyme in the absence of bound DNA, performed gel retardation analyses of DNA binding and bending, and have...

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Source

Identifiers

PMID: 10891275
DOI: 10.1006/jmbi.2000.3874

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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