Article
Alternative geometries of DNA looping: an analysis using the SfiI endonuclease.
Journal of molecular biology - 5 May 2000
Watson M A, Gowers D M, Halford S E
Abstract excerpt
Many processes are governed by proteins that bind to separate sites in DNA and loop out the intervening DNA, but the geometries of the loops have seldom been determined. The SfiI endonuclease cleaves DNA after interacting with two recognition sites, and is a favourable system for the analysis of DNA looping. A gel-shift assay was used here to examine the binding of SfiI to a series of linear DNA molecules...
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