Article
Functional analysis of Arg-308 mutants of Flp recombinase. Possible role of Arg-308 in coupling substrate binding to catalysis.
The Journal of biological chemistry - 15 Mar 1990
Parsons R L, Evans B R, Zheng L, Jayaram M
Abstract excerpt
The arginine residue at position 308 in the Flp recombinase corresponds to the only invariant arginine within the Int family of recombinases. Alterations of this residue result in Flp variants that retain substrate recognition, but form weaker protein-DNA complexes than wild type Flp. Furthermore, their DNA cleavage activity is significantly diminished. A conservative change of R308K results in a functional Flp...
Topics
- Arginine
- Bacterial Outer Membrane Proteins
- Base Sequence
- Catalysis
- DNA
- DNA Nucleotidyltransferases
- Escherichia coli
- Gene Expression
- Molecular Sequence Data
- Mutation
- Plasmids