Article
Rapid diagnosis of beta-thalassemia mutations in Chinese by naturally and amplified created restriction sites.
Blood - 15 Oct 1992
Chang J G, Chen P H, Chiou S S, Lee L S, Perng L I, Liu T C
Abstract excerpt
We developed a rapid and simple method to diagnose the molecular defects of beta-thalassemia in Chinese patients. This method involves the selective amplification of a DNA fragment from human beta globin gene with specific oligonucleotide primers, followed by digestion with restriction enzymes that recognize artificially created or naturally occurring restriction sites. To detect the 4-nucleotide deletion of...
Topics
- Base Sequence
- Codon
- DNA
- Deoxyribonuclease EcoRI
- Deoxyribonucleases, Type II Site-Specific
- Globins
- Humans
- Molecular Sequence Data
- Mutation
- Polymerase Chain Reaction
- Restriction Mapping