Article
Primer-introduced restriction analysis polymerase chain reaction method for non-invasive prenatal testing of β-thalassemia.
Hemoglobin - 1 Jan 2015
Liu Saijun, Chen Liyuan, Zhang Xiandong, Li Jian, Lin Haiying, Liu Louhui, Xie Jiansheng, Ge Huijuan, Ye Minglan, Chen Caifen, Ji Xingwen, Zhang Caifen, Xu Fengping, Jiang Hui, Zhen Hefu, Chen Shiping, Wang Wei
Abstract excerpt
We have developed a new method for non-invasive prenatal testing (NIPT) of paternally inherited fetal mutants for β-thalassemia (β-thal). Specially designed primer-introduced restriction analysis-polymerase chain reaction (PIRA-PCR) were used to detect four major mutations [IVS-II-654, HBB: c.316-197C > T; codon 17 (A > T), HBB: c.52A > T; -28 (A > G), HBB: c.-78A > G and codons 41/42 (-TTCT), HBB:...
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