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Article

Construction of adenovirus type 5 early region 1 and 4 virus mutants.

Methods in molecular medicine - 1 Jan 2007

Groitl Peter, Dobner Thomas

Abstract excerpt

This chapter describes a novel strategy that simplifies the generation and production of adenovirus type 5 (Ad5) mutants carrying defined mutations in early transcription units 1 (E1) and 4 (E4). The strategy involves three recombinant plasmids containing E1 (pE1-1235), E4 (pE4-1155), or the wild-type genome that lacks a portion of E3 (pH5pg4100). To generate recombinant viruses, mutations are first introduced...

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PMID: 17401162
DOI: 10.1385/1-59745-166-5:29

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