Article
Rapid characterization of mutations in amplified human hprt cDNA by polyacrylamide gel electrophoresis.
Mutation research - 1 Nov 1998
Garganta F, Krause G, Scherer G
Abstract excerpt
Compiling hprt mutation spectra involves the isolation and analysis of numerous 6-thioguanine-resistant clones for identifying characteristic point mutations. Since cDNA amplificates are compulsory intermediates in most mutant classification protocols, we suggest their preliminary characterizatio...
Topics
- Cell Line
- DNA, Complementary
- Deoxyribonucleases, Type II Site-Specific
- Electrophoresis, Polyacrylamide Gel
- Exons
- Humans
- Hypoxanthine Phosphoribosyltransferase
- Mutation
- Point Mutation
- Polymorphism, Single-Stranded Conformational
- Restriction Mapping
- Reverse Transcriptase Polymerase Chain Reaction
- Sensitivity and Specificity
- Sequence Deletion