Article
Identification of in vivo mutations in exon 5 of the human HPRT gene in a set of pooled T-cell mutants by constant denaturant capillary electrophoresis (CDCE).
Mutation research - 20 Jul 2000
Fält S, Kumar R, Wennborg A, Tomita-Mitchell A, Thilly W G, Lambert B
Abstract excerpt
Constant denaturant capillary electrophoresis (CDCE), based on co-operative DNA melting equilibria, has the resolving power to separate single nucleotide mutants from wild type sequences. We used this technique to study mutations in a 70-bp isomelting domain of the human HPRT gene, which included the entire exon 5 and its flanking splice donor and acceptor sites. Pooled samples of 6-thioguanine selected T-cell...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.