Article
Strand specificity for mutations induced by (+)-anti BPDE in the hprt gene in human T-lymphocytes.
Mutation research - 1 Sept 1992
Andersson B, Fält S, Lambert B
Abstract excerpt
Mutations in the hprt gene in T-lymphocyte clones isolated from primary cultures treated with the (+)-anti enantiomer of 7,8-dihydroxy-9,10-epoxy-7,8,9,10- tetrahydrobenzo[a]pyrene (BPDE) in vitro, and from untreated control cultures, were characterized using polymerase chain reaction and direct sequencing of hprt cDNA and genomic fragments. The spectrum of BPDE-induced mutations was very specific and clearly...
Topics
- 7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide
- Base Sequence
- Blotting, Southern
- DNA Repair
- DNA, Single-Stranded
- Humans
- Hypoxanthine Phosphoribosyltransferase
- Molecular Sequence Data
- Mutation
- Polymerase Chain Reaction