Article
Pyridoxal-5'-phosphate inhibits the polymerase activity of a recombinant RNAase H-deficient mutant of HIV-1 reverse transcriptase.
AIDS research and human retroviruses - 1 May 1992
Moen L K, Bathurst I C, Barr P J
Abstract excerpt
We have investigated the ability of pyridoxal-5'-phosphate to inhibit a recombinant deletion mutant of human immunodeficiency virus type 1(HIV-1) reverse transcriptase (RT) which is missing the last 23 amino acids of the C-terminus. This mutant reverse transcriptase is characterized by normal polymerase activity as compared with full-length enzyme; however, it has no RNase H activity. Inhibition studies with...
Topics
- DNA-Directed DNA Polymerase
- HIV-1
- Kinetics
- Magnesium
- Mutation
- Nucleic Acid Synthesis Inhibitors
- Pyridoxal Phosphate
- RNA-Directed DNA Polymerase
- Reverse Transcriptase Inhibitors
- Ribonuclease H