Article
Physiological Mg2+ Conditions Significantly Alter the Inhibition of HIV-1 and HIV-2 Reverse Transcriptases by Nucleoside and Non-Nucleoside Inhibitors in Vitro.
Biochemistry - 10 Jan 2017
Achuthan Vasudevan, Singh Kamlendra, DeStefano Jeffrey J
Abstract excerpt
Reverse transcriptases (RTs) are typically assayed in vitro with 5-10 mM Mg2+, whereas the free Mg2+ concentration in cells is much lower. Artificially high Mg2+ concentrations used in vitro can misrepresent different properties of human immunodeficiency virus (HIV) RT, including fidelity, catalysis, pausing, and RNase H activity. Here, we analyzed nucleoside (NRTIs) and non-nucleoside RT inhibitors (NNRTIs) in...
Topics
- Deoxycytosine Nucleotides
- Deoxyguanine Nucleotides
- Dideoxynucleotides
- Dose-Response Relationship, Drug
- Drug Interactions
- Electrophoresis, Polyacrylamide Gel
- HIV Reverse Transcriptase
- Humans
- Kinetics
- Magnesium
- Mutation
- Nucleosides
- Reverse Transcriptase Inhibitors