Article
A loss of viral replicative capacity correlates with altered DNA polymerization kinetics by the human immunodeficiency virus reverse transcriptase bearing the K65R and L74V dideoxynucleoside resistance substitutions.
The Journal of biological chemistry - 11 Jun 2004
Deval Jérôme, Navarro Jean-Marc, Selmi Boulbaba, Courcambeck Jérôme, Boretto Joëlle, Halfon Philippe, Garrido-Urbani Sarah, Sire Josephine, Canard Bruno
Abstract excerpt
Mechanisms governing viral replicative capacity are poorly understood at the biochemical level. Human immunodeficiency virus, type 1 reverse transcriptase (HIV-1 RT) K65R or L74V substitutions confer viral resistance to 2',3'-dideoxyinosine (ddI) in vivo. The two substitutions never occur together, and L74V is frequently found in patients receiving ddI, while K65R is not. Here we show that recombinant viruses...
Topics
- Cells, Cultured
- DNA Replication
- Deoxyadenine Nucleotides
- Didanosine
- Dideoxynucleotides
- Drug Resistance, Viral
- HIV Reverse Transcriptase
- Humans
- Kinetics
- Mutation
- Virus Replication