Article
Cloning, expression, purification, and biological activity of recombinant native and variant human alpha 1-antichymotrypsins.
The Journal of biological chemistry - 15 Jan 1990
Rubin H, Wang Z M, Nickbarg E B, McLarney S, Naidoo N, Schoenberger O L, Johnson J L, Cooperman B S
Abstract excerpt
Human alpha 1-antichymotrypsin has been cloned, sequenced and expressed in Escherichia coli and recombinant protein as well as point-specific mutants have been purified and characterized. The corrected gene-deduced amino acid sequence has 45% overall identity with alpha 1-protease inhibitor, which is higher than the 42% previously reported (Chandra, T., Stackhouse, R., Kidd, V. J., Robson, J. H., and Woo, S. L....
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