Article
Rapid isolation of human CD34 hematopoietic stem cells--purging of human tumor cells.
Transplantation - 1 May 1992
Lebkowski J S, Schain L R, Okrongly D, Levinsky R, Harvey M J, Okarma T B
Abstract excerpt
Human CD34+ hematopoietic stem cells were purified using a new technology in which monoclonal antibodies are covalently immobilized on polystyrene surfaces. The CD34+ cell isolation scheme involved three sequential processes: (1) purification of bone marrow mononuclear cells; (2) enrichment of CD...
Topics
- Antibodies, Monoclonal
- Antigens, CD
- Antigens, CD34
- Bone Marrow Cells
- Breast Neoplasms
- Carcinoma, Small Cell
- Cell Adhesion
- Cell Separation
- Flow Cytometry
- Fluorescent Antibody Technique
- Hematopoietic Stem Cells
- Humans
- Immunoglobulin G
- Lectins
- Leukemia, Myeloid, Acute
- Neuroblastoma
- Phenotype
- Plant Lectins