Article
Rapid isolation of human CD34 hematopoietic stem cells--purging of human tumor cells.
Transplantation - 1 May 1992
Lebkowski J S, Schain L R, Okrongly D, Levinsky R, Harvey M J, Okarma T B
Abstract excerpt
Human CD34+ hematopoietic stem cells were purified using a new technology in which monoclonal antibodies are covalently immobilized on polystyrene surfaces. The CD34+ cell isolation scheme involved three sequential processes: (1) purification of bone marrow mononuclear cells; (2) enrichment of CD34+ cells using covalently immobilized soybean agglutinin; and (3) positive selection of CD34+ cells using polystyrene...
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