Article
CD34 or S313 positive cells selection by avidin-biotin immunoadsorption.
Haematologica - 1 Mar 1991
Tassi C, Fortuna A, Bontadini A, Lemoli R M, Gobbi M, Tazzari P L
Abstract excerpt
A column immunoadsorption method, based on the high affinity between the protein Avidin and the vitamin Biotin has been used to obtain positive CD34+ or S313+ marrow cells selection. Cell suspensions from marrow samples of six healthy subjects were incubated either the monoclonal antibody S313 or HPCA 1 (My 10-like) and a biotinilated goat anti-mouse Immunoglobulins antiserum, passed over a column containing an...
Topics
- Antibodies, Monoclonal
- Antigens, CD
- Antigens, CD34
- Antigens, Differentiation
- Avidin
- Biotin
- Bone Marrow Cells
- Cell Separation
- Chromatography, Affinity
- Hematopoietic Stem Cells
- Humans
