Anti‐VEGFR‐2 scFvs for Cell Isolation. Single‐Chain Antibodies Recognizing the Human Vascular Endothelial Growth Factor Receptor‐2 (VEGFR‐2/flk‐1) on the Surface of Primary Endothelial Cells and Preselected CD34 + Cells from Cord Blood

Abstract excerpt

Five specific single-chain antibodies recognizing the human vascular endothelial growth factor receptor-2 (VEGFR-2/KDR) were selected from a V-gene phage display library constructed from mice immunized with the extracellular domain of VEGFR-2 (Ig-like domain 1-7). All five scFv antibodies (A2, A7, B11, G3, and H1) bound to the purified native antigen in enzyme-linked immunosorbent assay and Dot Blot, and showed...

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