Article
Establishment and characterization of an immortalized human sebaceous gland cell line (SZ95).
The Journal of investigative dermatology - 1 Dec 1999
Zouboulis C C, Seltmann H, Neitzel H, Orfanos C E
Abstract excerpt
Human facial sebaceous gland cells were transfected with a PBR-322-based plasmid containing the coding region for the Simian virus-40 large T antigen. The resulting proliferating cell cultures have been passaged over 50 times to date, have been cloned, and show no signs of senescence after 4&DF;1 2 y in vitro, whereas normal human sebocytes can only be grown for three to six passages. The immortalized transfected...
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