Article
The anticodon and discriminator base are major determinants of cysteine tRNA identity in vivo.
The Journal of biological chemistry - 15 Apr 1992
Pallanck L, Li S, Schulman L H
Abstract excerpt
Mutants of the Escherichia coli initiator tRNA (tRNA(fMet)) have been used to examine the role of the anticodon and discriminator base in in vivo aminoacylation of tRNAs by cysteinyl-tRNA synthetase. Substitution of the methionine anticodon CAU with the cysteine anticodon GCA was found to allow initiation of protein synthesis by the mutant tRNA from a complementary initiation codon in a reporter protein....
Topics
- Acylation
- Amino Acid Sequence
- Amino Acyl-tRNA Synthetases
- Anticodon
- Base Sequence
- DNA, Bacterial
- Escherichia coli
- Genetic Vectors
- Molecular Sequence Data
- Mutation
- Nucleic Acid Conformation