Article
The effects of cysteine mutations on the reverse transcriptases of human immunodeficiency virus types 1 and 2.
The Journal of biological chemistry - 15 Jan 1992
Hizi A, Shaharabany M, Tal R, Hughes S H
Abstract excerpt
Chemical modification of HIV-1 and HIV-2 (human immunodeficiency virus, types 1 and 2) reverse transcriptases (RT) with three thiol reactive compounds selectively inhibits the RNase H function of the enzyme. HIV-1 RT has 2 cysteines (at positions 38 and 280); HIV-2 RT has 3 (38, 280, 445). Both o...
Topics
- Cysteine
- DNA-Directed DNA Polymerase
- Electrophoresis, Polyacrylamide Gel
- Escherichia coli
- Gene Expression
- HIV Reverse Transcriptase
- HIV-1
- HIV-2
- Mutation
- RNA-Directed DNA Polymerase
- Reverse Transcriptase Inhibitors
- Ribonuclease H
- Sulfhydryl Compounds