Article
The proteinase yscA-inhibitor, IA3, gene. Studies of cytoplasmic proteinase inhibitor deficiency on yeast physiology.
FEBS letters - 20 May 1991
Schu P, Wolf D H
Abstract excerpt
The gene of the proteinase yscA inhibitor IA3, PAI3, of the yeast Saccharomyces cerevisiae was isolated by oligonucleotide screening of a genomic DNA library and sequenced. The gene codes for a single protein of 68 amino acids. The structural PAI3 gene was deleted in vitro by oligonucleotide-site-directed mutagenesis. The mutated allele was introduced via homologous recombination into the genome of wild-type...
Topics
- Amino Acid Sequence
- Base Sequence
- DNA, Fungal
- Genes, Fungal
- Molecular Sequence Data
- Mutation
- Nucleic Acid Hybridization
- Protease Inhibitors
- Saccharomyces cerevisiae
- Serine Endopeptidases
- Serine Proteinase Inhibitors