Article
Effects of proteinase A on cultivation and viability characteristics of industrial Saccharomyces cerevisiae WZ65.
Journal of Zhejiang University. Science. B - 1 Oct 2009
Zhang Hong-bo, Zhang Hai-feng, Chen Qi-he, Ruan Hui, Fu Ming-liang, He Guo-qing
Abstract excerpt
Proteinase A (PrA), encoded by PEP4 gene, is a key enzyme in the vacuoles of Saccharomyces cerevisiae. We characterized the effects of PrA on cell growth and glucose metabolism in the industrial S. cerevisiae WZ65. It was observed that the lag phase of cell growth of partial PEP4 gene deletion mutant (36 h) and PrA-negative mutant (48 h) was significantly extended, compared with the wild type strain (24 h)...
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