Article
Methods for preparation of recombinant cytokine proteins V. mutant analogues of human interferon-gamma with higher stability and activity.
Protein expression and purification - 1 Mar 2002
Pechenov Sergey E, Tikhonov Roman V, Shingarova Lyudmila N, Korobko Vyacheslav G, Yakimov Sergey A, Klyushnichenko Vadim E, Babajantz Alla A, Beliaev Dmitriy L, Kuznetzov Vladimir P, Shvetz Vitaliy I, Wulfson Andrey N
Abstract excerpt
Mutant analogues of recombinant human immune interferon (IFN-gamma) with higher stability and biological activity were prepared. Depending on the analogue, protein structure modification might involve introduction of an intramonomer disulfide bond (through replacements of Glu7Cys and Ser69Cys), C-terminal shortening by 10 amino acid residues, as well as Gln133Leu substitution in truncated variant. Isolation,...
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