Article
Generation of deletions in pTV1ts following transformation of Staphylococcus aureus protoplasts.
Letters in applied microbiology - 1 Feb 1991
Luchansky J B, Faith N G
Abstract excerpt
Plasmid pTV1ts was introduced into Staphylococcus aureus by transformation of protoplasts at frequencies ranging from 6.6 x 10(1) to 2.8 x 10(5) per micrograms of DNA when selection was made for resistance to chloramphenicol and erythromycin, respectively. Phenotypic analysis of regenerated transformants demonstrated three distinct classes. Analysis of the plasmid profiles of several isolates revealed that two...
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