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Article

Generation of deletions in pTV1ts following transformation of Staphylococcus aureus protoplasts.

Letters in applied microbiology - 1 Feb 1991

Luchansky J B, Faith N G

Abstract excerpt

Plasmid pTV1ts was introduced into Staphylococcus aureus by transformation of protoplasts at frequencies ranging from 6.6 x 10(1) to 2.8 x 10(5) per micrograms of DNA when selection was made for resistance to chloramphenicol and erythromycin, respectively. Phenotypic analysis of regenerated transformants demonstrated three distinct classes. Analysis of the plasmid profiles of several isolates revealed that two...

Topics

Chloramphenicol
Chromosome Deletion
DNA, Bacterial
Electrophoresis, Agar Gel
Erythromycin
Phenotype
Plasmids
Protoplasts
Restriction Mapping
Staphylococcus aureus
Transformation, Genetic

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Identifiers

PMID: 1367001
DOI: 10.1111/j.1472-765x.1991.tb00500.x

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1367001-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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