Article
Long regions of homologous DNA are incorporated into the tobacco plastid genome by transformation.
The Plant cell - 1 Jan 1992
Staub J M, Maliga P
Abstract excerpt
We investigated the size of flanking DNA incorporated into the tobacco plastid genome alongside a selectable antibiotic resistance mutation. The results showed that integration of a long uninterrupted region of homologous DNA, rather than of small fragments as previously thought, is the more likely event in plastid transformation of land plants. Transforming plasmid pJS75 contains a 6.2-kb DNA fragment from the...
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