Article
[Mutations of ras and p53 genes in human non-small cell lung cancer cell lines and their clinical significance].
Nihon Geka Gakkai zasshi - 1 Sept 1992
Mitsudomi T, Oyama T, Gazdar A F, Minna J D, Okabayashi K, Shirakusa T
Abstract excerpt
We examined 77 non-small cell lung cancer (NSCLC) cell lines for mutations of 3 ras genes and p53 gene, and ras mutations were detected by designed RFLP assay generated by mismatched primers during the PCR step and p53 gene mutations were detected using SSCP analysis. The incidence of ras mutations were 27/77 (35%) while that of p53 gene mutations were 57/77 (74%). The incidence of ras mutations in cell lines...
Topics
- Carcinoma, Non-Small-Cell Lung
- Genes, p53
- Genes, ras
- Humans
- Lung Neoplasms
- Mutation
- Polymerase Chain Reaction
- Polymorphism, Restriction Fragment Length
- Tumor Cells, Cultured