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Article

Combined use of RFLP and PCR-ASO typing for HLA-DR-Dw and DQw typing.

European journal of immunogenetics : official journal of the British Society for Histocompatibility and Immunogenetics - 1 Jan 2000

Bignon J D, Bidwell J L

Abstract excerpt

Due to some limitations of restriction fragment length polymorphism (RFLP) analysis in HLA-DR-DQ typing, we present a combined use of RFLP and polymerase chain reaction (PCR)-allele-specific oligonucleotide (ASO) typing. This scheme consists in selectively amplifying the few RFLP ill-defined genes (DR1/DR'Br' and DR4-Dw subsets) using PCR with allele specific primers to avoid cross-hybridization.

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Source

Identifiers

PMID: 1676910
DOI: 10.1111/j.1744-313x.1991.tb00012.x

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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