Article
Antibody-resistant mutants of Borrelia burgdorferi: in vitro selection and characterization.
The Journal of experimental medicine - 1 Sept 1992
Sădziene A, Rosa P A, Thompson P A, Hogan D M, Barbour A G
Abstract excerpt
We used polyclonal antisera and monoclonal antibodies (mAbs) to inhibit the growth of clonal populations of two strains of Borrelia burgdorferi, the Lyme disease agent, and thereby select for antibody-resistant mutants. mAbs were directed at the outer membrane proteins, OspA or OspB. Mutants resi...
Topics
- Amino Acid Sequence
- Antibodies, Bacterial
- Base Sequence
- Blotting, Southern
- Blotting, Western
- Borrelia burgdorferi Group
- DNA, Bacterial
- Molecular Sequence Data
- Mutation
- Phenotype