Article
An amino-terminal fragment of SV40 T antigen transforms REF52 cells.
Virology - 1 Nov 1992
Sompayrac L, Danna K J
Abstract excerpt
An SV40 mutant, T147D, encodes only the amino-terminal 147 amino acids of large T antigen and does not make small t antigen. We show here that a retrovirus which expresses this mutant T antigen transforms rat REF52 cells as efficiently as a retrovirus that expresses both the wild-type large and small T antigens. This cell line had previously been refractory to transformation by mutants that make short,...
Topics
- Animals
- Antigens, Polyomavirus Transforming
- Cell Line
- Cell Transformation, Viral
- Cloning, Molecular
- Mutation
- Peptide Fragments
- Rats
- Simian virus 40
- Transfection