Construction of a US3 lacZ insertion mutant of herpes simplex virus type 2 and characterization of its phenotype in vitro and in vivo.

doi:10.1016/0042-6822(92)91212-d

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Article

Construction of a US3 lacZ insertion mutant of herpes simplex virus type 2 and characterization of its phenotype in vitro and in vivo.

Virology - 1 Sept 1992

Nishiyama Y, Yamada Y, Kurachi R, Daikoku T

Abstract excerpt

We have constructed and characterized a mutant of herpes simplex virus type 2 (HSV-2) which was inserted a modified lacZ gene, placed under the control of HSV-1 beta 8 promotor, into the US3 protein kinase gene. The mutant, L1BR1, could not induce the virus-encoded protein kinase activity, but co...

Topics

Aging
Animals
Cell Division
Chromatography, DEAE-Cellulose
Cloning, Molecular
Gene Expression Regulation, Viral
Herpes Simplex
Kinetics
L Cells
Lac Operon
Macrophages
Mice

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Mutagenesis, Insertional

Organ Specificity

Phenotype

Protein Kinases

Restriction Mapping

Simplexvirus

Source

Open in PubMed

Identifiers

PMID: 1326804
DOI: 10.1016/0042-6822(92)91212-d

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-1326804-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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