Article
Expression and characterization of recombinant human protein S in heterologous cells--studies of the interaction of amino acid residues leu-608 to glu-612 with human C4b-binding protein.
Thrombosis and haemostasis - 4 May 1992
Chang G T, Ploos van Amstel H K, Hessing M, Reitsma P H, Bertina R M, Bouma B N
Abstract excerpt
Mouse C127 epithelioid cells were genetically engineered to produce biologically active gamma-carboxylated human protein S. A full length human protein S cDNA was cloned into a bovine papilloma virus (BPV) based shuttle vector under the transcriptional control of the Moloney murine sarcoma virus...
Topics
- Amino Acid Sequence
- Animals
- Base Sequence
- Blood Proteins
- Carrier Proteins
- Cells, Cultured
- Complement Inactivator Proteins
- DNA Transposable Elements
- Genetic Variation
- Genetic Vectors
- Glutamine
- Glycoproteins
- Humans
- Leucine
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Protein Binding
- Protein S