Expression and characterization of recombinant human factor V and a mutant lacking a major portion of the connecting region.

doi:10.1021/bi00481a003

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Article

Expression and characterization of recombinant human factor V and a mutant lacking a major portion of the connecting region.

Biochemistry - 24 Jul 1990

Kane W H, Devore-Carter D, Ortel T L

Abstract excerpt

Human coagulation factor V is a protein cofactor that is an essential component of the prothrombinase complex. A full-length factor V cDNA has been subcloned into the mammalian expression vector pDX and used to transfect COS cells. Approximately 95 +/- 4% of the recombinant human factor V (rHFV)...

Topics

Base Sequence
Cloning, Molecular
DNA
Factor V
Gene Expression
Genetic Vectors
Humans
Molecular Sequence Data
Mutation
Recombinant Proteins

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