Article
The mechanism of somatic inhibition of Drosophila P-element pre-mRNA splicing: multiprotein complexes at an exon pseudo-5' splice site control U1 snRNP binding.
Genes & development - 1 Aug 1992
Siebel C W, Fresco L D, Rio D C
Abstract excerpt
Somatic inhibition restricts splicing of the Drosophila P-element third intron (IVS3) to the germ line. We have exploited this simple system to provide a model for a mechanism of alternative pre-mRNA splicing. Biochemical complementation experiments revealed that Drosophila somatic extracts inhibited U1 snRNP binding to the 5' splice site. Using sensitive RNase protection and modification-interference assays, we...
Topics
- Animals
- Base Sequence
- Binding Sites
- DNA Transposable Elements
- Drosophila
- Introns
- Macromolecular Substances
- Molecular Sequence Data
- Mutation
- RNA Precursors
- RNA Splicing