Article
Cloning and expression of gene 4 of bacteriophage T7 and creation and analysis of T7 mutants lacking the 4A primase/helicase or the 4B helicase.
The Journal of biological chemistry - 25 Jul 1992
Rosenberg A H, Patel S S, Johnson K A, Studier F W
Abstract excerpt
T7 gene 4, which is required for DNA replication, specifies two proteins whose coding sequences overlap in the same reading frame: the 4A protein, a 566-amino acid primase/helicase, and the 4B protein, a 503-amino acid helicase whose initiation codon is the 64th codon of the 4A protein. To study...
Topics
- Base Sequence
- Blotting, Western
- Cloning, Molecular
- DNA Helicases
- DNA Primase
- DNA Replication
- Electrophoresis, Polyacrylamide Gel
- Gene Expression
- Genes, Viral
- Isoenzymes
- Molecular Sequence Data
- Mutation
- Plasmids
- RNA Nucleotidyltransferases
- Restriction Mapping
- T-Phages
- Viral Proteins
- Virus Replication