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Article

Expression of an active Na,K-ATPase with an alpha-subunit lacking all twenty-three native cysteine residues.

The Journal of biological chemistry - 29 Sept 2000

Hu Y K, Eisses J F, Kaplan J H

Abstract excerpt

We have constructed a mutant Na,K-ATPase alpha1-subunit with all native cysteine residues replaced. Using the baculovirus system, this cysteine-less alpha1-subunit and wild-type beta1-subunit were expressed in High Five cells. After 3 days of infection, cells were fractionated, and endoplasmic reticulum, Golgi apparatus, and plasma membranes were isolated. The molecular activity of the cysteine-less mutant in the...

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PMID: 10906129
DOI: 10.1074/jbc.M003737200

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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