Article
Functional consequences of substitution of the disulfide-bonded segment, Cys127-Cys150, located in the extracellular domain of the Na,K-ATPase beta subunit: Arg148 is essential for the functional expression of Na,K-ATPase.
Journal of biochemistry - 1 Mar 1995
Ueno S, Kusaba M, Takeda K, Maeda M, Futai M, Izumi F, Kawamura M
Abstract excerpt
The Cys127-Cys150 disulfide-bonded loop (L1) of the Torpedo californica Na,K-ATPase beta 1 subunit was substituted with the corresponding loop of the rat beta 1, mouse beta 2, or pig H,K-ATPase beta subunit. All the substituted mutant beta subunits assembled with the Na,K-ATPase alpha subunit in a trypsin-resistant manner. The mutants with L1 from the Na,K-ATPase beta subunit isoforms (rat beta 1 and mouse beta...
Topics
- Amino Acid Sequence
- Animals
- Arginine
- Cysteine
- Disulfides
- Hydrolysis
- Mice
- Molecular Sequence Data
- Mutation
- Oocytes
- Protein Structure, Tertiary