An Escherichia coli DNA topoisomerase I mutant has a compensatory mutation that alters two residues between functional domains of the DNA gyrase A protein.

doi:10.1128/jb.174.12.4175-4178.1992

Article

An Escherichia coli DNA topoisomerase I mutant has a compensatory mutation that alters two residues between functional domains of the DNA gyrase A protein.

Journal of bacteriology - 1 Jun 1992

Oram M, Fisher L M

Abstract excerpt

Nucleotide sequence analysis revealed that the compensatory gyrA mutation in Escherichia coli DM750 affects DNA supercoiling by interchanging the identities of Ala-569 and Thr-586 in the DNA gyrase A subunit. These residues flank Arg-571, a site for trypsin cleavage that splits gyrase A protein b...

Topics

Amino Acid Sequence
Base Sequence
DNA Topoisomerases, Type I
DNA Topoisomerases, Type II
DNA, Superhelical
Escherichia coli
Molecular Sequence Data
Mutation
Oligodeoxyribonucleotides
Polymerase Chain Reaction
Restriction Mapping

An Escherichia coli DNA topoisomerase I mutant has a compensatory mutation that alters two residues between functional domains of the DNA gyrase A protein.

Abstract excerpt

Topics

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