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Article

Probing the limits of the DNA breakage-reunion domain of the Escherichia coli DNA gyrase A protein.

The Journal of biological chemistry - 25 Feb 1991

Reece R J, Maxwell A

Abstract excerpt

In a previous report (Reece, R. J., and Maxwell, A. (1989) J. Biol. Chem. 264, 19648-19653) we showed that treatment of the Escherichia coli DNA gyrase A protein with trypsin generates two stable fragments. The N-terminal 64-kDa fragment supports DNA supercoiling, while the C-terminal 33-kDa fragment shows no enzymic activity. We proposed that the 64-kDa fragment represents the DNA breakage-reunion domain of the...

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PMID: 1847377

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