Probing the limits of the DNA breakage-reunion domain of the Escherichia coli DNA gyrase A protein.

Article

Probing the limits of the DNA breakage-reunion domain of the Escherichia coli DNA gyrase A protein.

The Journal of biological chemistry - 25 Feb 1991

Reece R J, Maxwell A

Abstract excerpt

In a previous report (Reece, R. J., and Maxwell, A. (1989) J. Biol. Chem. 264, 19648-19653) we showed that treatment of the Escherichia coli DNA gyrase A protein with trypsin generates two stable fragments. The N-terminal 64-kDa fragment supports DNA supercoiling, while the C-terminal 33-kDa frag...

Topics

Cloning, Molecular
DNA Damage
DNA Topoisomerases, Type II
Electrophoresis, Polyacrylamide Gel
Escherichia coli
Mutation
Plasmids
Protein Engineering
Quinolones

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

discussions

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.