Article
Mechanism of action of a repressor of dioxin-dependent induction of Cyp1a1 gene transcription.
Molecular and cellular biology - 1 May 1992
Watson A J, Weir-Brown K I, Bannister R M, Chu F F, Reisz-Porszasz S, Fujii-Kuriyama Y, Sogawa K, Hankinson O
Abstract excerpt
A dominant mutant of Hepa-1 cells, c31, expresses a repressor that prevents 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)-dependent stimulation of Cyp1a1 transcription. The repressor acts via the xenobiotic-responsive elements (XREs), which are the DNA-binding sites for the aryl hydrocarbon (Ah) receptor-TCDD complex during transcriptional activation of the gene. High-salt nuclear extracts prepared from c31 cells...
Topics
- Animals
- Base Sequence
- Binding Sites
- Cell Line
- Chimera
- Chloramphenicol O-Acetyltransferase
- Cytochrome P-450 Enzyme System
- DNA, Neoplasm
- Enzyme Induction
- Enzyme Repression
- Genes, Dominant