Article
Inhibition of the cellular actions of nerve growth factor by staurosporine and K252A results from the attenuation of the activity of the trk tyrosine kinase.
Biochemistry - 28 Apr 1992
Ohmichi M, Decker S J, Pang L, Saltiel A R
Abstract excerpt
The protein kinase inhibitors staurosporine and K252A inhibit some of the cellular actions of nerve growth factor (NGF). To explore the molecular mechanisms involved, we test the ability of these agents to block one of the earliest cellular responses to NGF, protein tyrosine phosphorylation. Concentrations of 10-100 nM staurosporine and K252A inhibit NGF-dependent tyrosine phosphorylation in PC12 cells and...
Topics
- Alkaloids
- Carbazoles
- Cell Line, Transformed
- Humans
- Indole Alkaloids
- Nerve Growth Factors
- Oncogene Proteins
- Phenotype
- Phosphorylation
- Protein-Tyrosine Kinases
- Receptors, Cell Surface