Article
p53: a transdominant regulator of transcription whose function is ablated by mutations occurring in human cancer.
The EMBO journal - 1 Apr 1992
Unger T, Nau M M, Segal S, Minna J D
Abstract excerpt
Gal4-p53 fusion constructs demonstrate that wild type p53 is a potent transactivator in human lung cancer cells with the transactivation domain for p53 residing in amino acids 1-42. Strikingly, a variety of lung cancer derived p53 mutations occurring outside this domain disrupt this activity. Temperature sensitive conformational shifts of p53 mutant proteins to the wild type form exist and, with a temperature...
Topics
- Amino Acid Sequence
- Carcinoma, Non-Small-Cell Lung
- Carcinoma, Small Cell
- Cell Line
- Chimera
- Chloramphenicol O-Acetyltransferase
- Chromosome Deletion
- DNA-Binding Proteins
- Fungal Proteins
- Gene Expression Regulation, Neoplastic