Article
Rescue of erythroid development in gene targeted GATA-1- mouse embryonic stem cells.
Nature genetics - 1 May 1992
Simon M C, Pevny L, Wiles M V, Keller G, Costantini F, Orkin S H
Abstract excerpt
Development of definitive (fetal liver-derived) red cells is blocked by a targeted mutation in the gene encoding the transcription factor GATA-1. We used in vitro differentiation of GATA-1- mouse embryonic stem (ES) cells to reveal a requirement for GATA-1 during primitive (yolk sac-derived) erythropoiesis and to establish a rescue assay. We show that the block to development includes primitive, as well as...
Topics
- Animals
- Cell Differentiation
- Cells, Cultured
- DNA-Binding Proteins
- Embryo, Mammalian
- Erythroid-Specific DNA-Binding Factors
- Erythropoiesis
- GATA1 Transcription Factor
- Humans
- Mice
- Mutation