Evaluation of Real-Time PCR for Detection of and Discrimination between <i>Bordetella pertussis</i> , <i>Bordetella parapertussis</i> , and <i>Bordetella holmesii</i> for Clinical Diagnosis

Abstract excerpt

PCR is increasingly being used as a diagnostic test for the detection of Bordetella pertussis and Bordetella parapertussis DNA, as it has improved sensitivity and specificity in comparison to conventional techniques. The assay described here uses the two insertion sequences IS481 and IS1001 for B. pertussis and B. parapertussis, respectively, with detection by molecular beacons. The real-time PCR for IS481...

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